Nicholas E. Manicke
Associate Professor
Department of Chemistry and Chemical Biology
Forensic and Investigative Sciences Program
Indiana University-Purdue University Indianapolis
402 N. Blackford St.
Indianapolis, IN

Contact Information:
O: 317-274-6819
E: nmanicke@iupui.edu

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Peer-reviewed Research Publications
*corresponding author
undergraduate researcher

Indianapolis
Nguyen CB, Wichert WRA, Carmany  DO, McBride EM, Mach PM,    Dhummakupt ES, Glaros T, and Manicke NE*: Pressure-Sensitive Adhesive Combined with Paper Spray Mass Spectrometry for Low-Cost Collection and Analysis of Drug Residues. Analytical Chemistry. In Press (2021) https://doi.org/10.1021/acs.analchem.1c02050

Skaggs C, Kirkpatrick L, Nguyen C, Dowling S, Zimmerman H, Ren G, and Manicke NE*: Simultaneous optimization of paper spray substrates and solvents for hydrophilic and hydrophobic molecules. International Journal of Mass Spectrometry. 470, 116705 (2021). Special Issue: Recent Developments in Ambient Ionization. https://doi.org/10.1016/j.ijms.2021.116705

Masterson AN, Hati S, Ren G, Liyanage T, Manicke NE, Goodpaster JV, and Sardar R*: Enhancing Nonfouling and Sensitivity of Surface-Enhanced Raman Scattering Substrates for Potent Drug Analysis in Blood Plasma via Fabrication of a Flexible Plasmonic Patch. Analytical Chemistry. 93 (4), 2578-2588 (2021) . https://pubs.acs.org/doi/10.1021/acs.analchem.0c04643

Bills BJ and Manicke NE*: Using Sesame Seed Oil to Preserve and Preconcentrate Cannabinoids for Paper Spray Mass Spectrometry. Journal of the American Society for Mass Spectrometry. 31 (3), 675–684 (2020) . https://pubs.acs.org/doi/abs/10.1021/jasms.9b00113

Skaggs CL, Ren GJ, Shi R.Z., El Gierari ETM, Manicke NE and Kirkpatrick LM*: Simultaneous Quantitation of Five Triazole Anti-fungal Agents by Paper Spray-Mass Spectrometry. Clinical Chemistry and Laboratory Medicine. 58(5), 836-846 (2020) . https://doi.org/10.1515/cclm-2019-0895

Skaggs CL, Kirkpatrick LM, Wichert WRA, Skaggs N, and Manicke NE*: A Statistical Approach to Optimizing Paper Spray Mass Spectrometry Parameters. Rapid Communications in Mass Spectrometry. 34, e8601 (2020). https://doi.org/10.1002/rcm.8601

Liyanage T, Masterson N, Hati S, Ren G, Manicke NE.  Rusynia D E, and Sardar R., Optimization of electromagnetic hot spots in surface-enhanced Raman scattering substrates for an ultrasensitive drug assay of emergency department patients' plasma. Analyst. 145 (23), 7662-7672 (2020). https://doi.org/10.1039/D0AN01372B

Wichert WRA, Dhummakupt ES, Zhang C, Mach PM, Bernhards RC, Glaros T, and Manicke NE*: Detection of Protein Toxin Simulants from Contaminated Surfaces by Paper Spray Mass Spectrometry. Journal of the American Society for Mass Spectrometry. Focus Issue: Emerging Investigators. 30, 1406-1415 (2019). https://doi.org/10.1007/s13361-019-02141-6

Dowling S, McBride EM, McKenna J, Glaros T, Manicke NE*: Direct soil analysis by paper spray mass spectrometry: Detection of drugs and chemical warfare agent hydrolysis products. Forensic Chemistry. 17, 100206 (2019). https://doi.org/10.1016/j.forc.2019.100206

Owings CG, Banerjee A, Asher TM, Gilhooly WP, Tuceryan A, Huffine M, Skaggs CL, Adebowale IM, Manicke NE, and Picard CJ*. Female Blow Flies As Vertebrate Resource Indicators. Scientific Reports. 9, 10594 (2019). https://doi.org/10.1038/s41598-019-46758-9

McKenna J, Shanks K, and Manicke NE*: Toxicological Drug Screening using Paper Spray High-Resolution Tandem Mass Spectrometry (HR-MS/MS). Journal of Analytical Toxicology. 42 (5), 300-310 (2018). https://doi.org/10.1093/jat/bky001

Bills BJ, Kinkade J, Ren G, and Manicke NE*: The Impacts of Paper Properties on Matrix Effects During Paper Spray Mass Spectrometry Analysis of Prescription Drugs, Fentanyl and Synthetic Canabinoids. Forensic Chemistry. 11, 15-22 (2018). https://doi.org/10.1016/j.forc.2018.08.002

Owings CG, Skaggs C, Sheriff W, Manicke NE, Picard CJ. Chemical Assay for the Detection of Vertebrate Fecal Metabolites in Adult Blow Flies (Diptera: Calliphoridae). Environmental Entomology. 47 (5), 586-593 (2018). https://doi.org/10.1093/ee/nvy027

Zhang C, Glaros T, and Manicke NE*: Targeted Protein Detection using an All-In-One Mass Spectrometry Cartridge. Journal of the American Chemical Society. 139 (2), 10996-10999 (2017). http://dx.doi.org/10.1021/jacs.7b05571

Fedick PW, Bills BJ, Manicke NE, and Cooks RG: Forensic Sampling and Analysis from a Single Substrate: Surface-Enhanced Raman Spectroscopy Followed by Paper Spray Mass Spectrometry. Analytical Chemistry. 89 (20), 10973-10979 (2017). http://dx.doi.org/10.1021/acs.analchem.7b02798

Dhummakupt ES, Mach PM, Carmany D, Demond PS, Moran TS, Connell T, Wylie HS, Manicke NE, Nilles JM, and Glaros T: Direct Analysis of Aerosolized Chemical Warfare Simulants Captured on a Modified Glass-Based Substrate by “Paper-Spray” Ionization. Analytical Chemistry. 89 (20), 10866-10872 (2017). http://dx.doi.org/10.1021/acs.analchem.7b02530

Jett R, Skaggs C, and Manicke NE*: Drug Screening Method Development for Paper Spray Coupled to a Triple Quadrupole Mass Spectrometer. Analytical Methods. 9, 5037-5043 (2017). http://dx.doi.org/10.1039/C7AY01009E

McKenna J, Dhummakupt ES, Connell T, Demond PS, Miller DB, Nilles JM, Manicke NE* and Glaros T*: Detection of Chemical Warfare Agent Simulants and Hydrolysis Products in Biological Samples by Paper Spray Mass Spectrometry. Analyst. 142, 1442-1451 (2017). http://dx.doi.org/10.1039/C7AN00144D

Bills BJ and Manicke NE*: Development of a Prototype Blood Fractionation Cartridge for Plasma Analysis by Paper Spray Mass Spectrometry. Clinical Mass Spectrometry 2, 18-24 (2016). http://dx.doi.org/10.1016/j.clinms.2016.12.002

Vega C, Spence C, Zhang C, Bills BJ, Manicke NE*: Ionization Suppression and Recovery in Direct Biofluid Analysis Using Paper Spray Mass Spectrometry. Journal of the American Society of Mass Spectrometry 27(4), 726-734 (2016). http://dx.doi.org/10.1007/s13361-015-1322-8

Shi R-Z, El Gierari ETM, Faix JD, Manicke NE*: Rapid Measurement of Cyclosporine and Sirolimus in Whole Blood by Paper Spray-Tandem Mass Spectrometry. Clinical Chemistry 62 (1), 295-297 (2016). http://dx.doi.org/10.1373/clinchem.2015.245191

Zhang C and Manicke NE*: Development of a Paper Spray Mass Spectrometry Cartridge with Integrated Solid Phase Extraction for Bioanalysis. Analytical Chemistry 87(12), 6212-6219 (2015). http://dx.doi.org/10.1021/acs.analchem.5b00884

Manicke NE* and Belford M: Separation of Opiate Isomers Using Electrospray Ionization and Paper Spray Coupled to High-Field Asymmetric Waveform Ion Mobility Spectrometry. Journal of the American Society for Mass Spectrometry 26(5), 701-705 (2015). http://dx.doi.org/10.1007/s13361-015-1096-z

Shi R-Z, El Gierari ETM, Manicke NE*, Faix JD: Rapid Measurement of Tacrolimus in Whole Blood by Paper Spray-Tandem Mass Spectrometry (PS-MS/MS). Clinica Chimica Acta 441, 99-104 (2015). http://dx.doi.org/10.1016/j.cca.2014.12.022

West Lafayette
Espy RD, Teunissen SF, Manicke NE, Ren Y, Ouyang Z, Van Asten A, Cooks RG: Paper Spray and Extraction Spray Mass Spectrometry for the Direct and Simultaneous Quantification of Eight Drugs of Abuse in Whole Blood. Analytical Chemistry 86(15), 7712-7718 (2014). http://dx.doi.org/10.1021/ac5016408

Shen LH, Zhang J, Yang Q, Manicke NE, Ouyang Z: High Throughput Paper Spray Mass Spectrometry Analysis. Clinica Chimica Acta 420, 28-33 (2013). http://dx.doi.org/10.1016/j.cca.2012.10.025

Wang H, Ren Y, Mcluckey MN, Manicke NE, Park J, Zheng LX, Shi RY, Cooks RG, Ouyang Z: Direct Quantitative Analysis of Nicotine Alkaloids from Biofluid Samples Using Paper Spray Mass Spectrometry. Analytical Chemistry 85(23), 11540-11544 (2013). http://dx.doi.org/10.1021/ac402798m

Espy RD, Manicke NE, Ouyang Z, Cooks RG: Rapid Analysis of Whole Blood by Paper Spray Mass Spectrometry for Point-of-Care Therapeutic Drug Monitoring. Analyst 137(10), 2344-2349 (2012). http://dx.doi.org/10.1039/c2an35082c

Yang Q, Manicke NE, Wang H, Petucci C, Cooks RG, Ouyang Z: Direct and Quantitative Analysis of Underivatized Acylcarnitines in Serum and Whole Blood Using Paper Spray Mass Spectrometry. Analytical and Bioanalytical Chemistry 404(5), 1389-1397 (2012). http://dx.doi.org/10.1007/s00216-012-6211-4

Yang Q, Wang H, Maas JD, Chappell WJ, Manicke NE, Cooks RG, Ouyang Z: Paper Spray Ionization Devices for Direct, Biomedical Analysis Using Mass Spectrometry. International Journal of Mass Spectrometry 312, 201-207 (2012). http://dx.doi.org/10.1016/j.ijms.2011.05.013

 Zhang ZP, Xu W, Manicke NE, Cooks RG, Ouyang Z: Silica Coated Paper Substrate for Paper-Spray Analysis of Therapeutic Drugs in Dried Blood Spots. Analytical Chemistry 84(2), 931-938 (2012). http://dx.doi.org/10.1021/ac202058w

Cooks RG, Manicke NE, Dill AL, Ifa DR, Eberlin LS, Costa AB, Wang H, Huang GM, Zheng OY: New Ionization Methods and Miniature Mass Spectrometers for Biomedicine: Desi Imaging for Cancer Diagnostics and Paper Spray Ionization for Therapeutic Drug Monitoring. Faraday Discussions 149, 247-267 (2011). http://dx.doi.org/10.1039/c005327a

Manicke NE, Abu-Rabie P, Spooner N, Ouyang Z, Cooks RG: Quantitative Analysis of Therapeutic Drugs in Dried Blood Spot Samples by Paper Spray Mass Spectrometry: An Avenue to Therapeutic Drug Monitoring. Journal of the American Society for Mass Spectrometry 22(9), 1501-1507 (2011). http://dx.doi.org/10.1007/s13361-011-0177-x
*One of the 3 most cited articles in JASMS since 2010 (as of July 2018).

Manicke NE, Yang QA, Wang H, Oradu S, Ouyang Z, Cooks RG: Assessment of Paper Spray Ionization for Quantitation of Pharmaceuticals in Blood Spots. International Journal of Mass Spectrometry 300(2-3), 123-129 (2011). http://dx.doi.org/10.1016/j.ijms.2010.06.037
*One of the 3 most cited articles in IJMS since 2010 (as of July 2018).

Wang H, Manicke NE, Yang QA, Zheng LX, Shi RY, Cooks RG, Zheng OY: Direct Analysis of Biological Tissue by Paper Spray Mass Spectrometry. Analytical Chemistry 83(4), 1197-1201 (2011). http://dx.doi.org/10.1021/ac103150a

Liu JJ, Wang H, Manicke NE, Lin JM, Cooks RG, Ouyang Z: Development, Characterization, and Application of Paper Spray Ionization. Analytical Chemistry 82(6), 2463-2471 (2010). http://dx.doi.org/10.1021/ac902854g

Manicke NE, Dill AL, Ifa DR, Cooks RG: High-Resolution Tissue Imaging on an Orbitrap Mass Spectrometer by Desorption Electrospray Ionization Mass Spectrometry. Journal of Mass Spectrometry 45(2), 223-226 (2010). http://dx.doi.org/10.1002/jms.1707

Wu CP, Ifa DR, Manicke NE, Cooks RG: Molecular Imaging of Adrenal Gland by Desorption Electrospray Ionization Mass Spectrometry. Analyst 135(1), 28-32 (2010). http://dx.doi.org/10.1039/b919816d

 Xu W, Manicke NE, Cooks GR, Ouyang Z: Miniaturization of Mass Spectrometry Analysis Systems. JALA 15(6), 433-439 (2010). http://dx.doi.org/10.1016/j.jala.2010.06.004

Dill AL, Ifa DR, Manicke NE, Costa AB, Ramos-Vara JA, Knapp DW, Cooks RG: Lipid Profiles of Canine Invasive Transitional Cell Carcinoma of the Urinary Bladder and Adjacent Normal Tissue by Desorption Electrospray Ionization Imaging Mass Spectrometry. Analytical Chemistry 81(21), 8758-8764 (2009). http://dx.doi.org/10.1021/ac901028b

Dill AL, Ifa DR, Manicke NE, Zheng OY, Cooks RG: Mass Spectrometric Imaging of Lipids Using Desorption Electrospray Ionization. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences 877(26), 2883-2889 (2009). http://dx.doi.org/10.1016/j.jchromb.2008.12.058

Manicke NE, Kistler T, Ifa DR, Cooks RG, Ouyang Z: High-Throughput Quantitative Analysis by Desorption Electrospray Ionization Mass Spectrometry. Journal of the American Society for Mass Spectrometry 20(2), 321-325 (2009). http://dx.doi.org/10.1016/j.jasms.2008.10.011

Manicke NE, Nefliu M, Wu CP, Woods JW, Reiser V, Hendrickson RC, Cooks RG: Imaging of Lipids in Atheroma by Desorption Electrospray Ionization Mass Spectrometry. Analytical Chemistry 81(21), 8702-8707 (2009). http://dx.doi.org/10.1021/ac901739s

Wu CP, Ifa DR, Manicke NE, Cooks RG: Rapid, Direct Analysis of Cholesterol by Charge Labeling in Reactive Desorption Electrospray Ionization. Analytical Chemistry 81(18), 7618-7624 (2009). http://dx.doi.org/10.1021/ac901003u

Ifa DR, Manicke NE, Dill AL, Cooks G: Latent Fingerprint Chemical Imaging by Mass Spectrometry. Science 321(5890), 805-805 (2008). http://dx.doi.org/10.1126/science.1157199

Ifa DR, Manicke NE, Rusine AL, Cooks RG: Quantitative Analysis of Small Molecules by Desorption Electrospray Ionization Mass Spectrometry from Polytetrafluoroethylene Surfaces. Rapid Communications in Mass Spectrometry 22(4), 503-510 (2008). http://dx.doi.org/10.1002/rcm.3377

Manicke NE, Wiseman JM, Ifa DR, Cooks RG: Desorption Electrospray Ionization (Desi) Mass Spectrometry and Tandem Mass Spectrometry (Ms/Ms) of Phospholipids and Sphingolipids: Ionization, Adduct Formation, and Fragmentation. Journal of the American Society for Mass Spectrometry 19(4), 531-543 (2008). http://dx.doi.org/10.1016/j.jasms.2007.12.003

Wiseman JM, Ifa DR, Zhu YX, Kissinger CB, Manicke NE, Kissinger PT, Cooks RG: Desorption Electrospray Ionization Mass Spectrometry: Imaging Drugs and Metabolites in Tissues. Proceedings of the National Academy of Sciences of the United States of America 105(47), 18120-18125 (2008). http://dx.doi.org/10.1073/pnas.0801066105

Ifa DR, Gumaelius LM, Eberlin LS, Manicke NE, Cooks RG: Forensic Analysis of Inks by Imaging Desorption Electrospray Ionization (Desi) Mass Spectrometry. Analyst 132(5), 461-467 (2007). http://dx.doi.org/10.1039/b700236j

Refereed Reviews, Book Chapters, and Editorials

McBride EM, Mach PM, Dhummakupt ES, Dowling S, Carmany DO, Demond PS, Rizzo G, .Manicke NE, and Glaros, T. Paper Spray Ionization: Applications and Perspectives. TRAC - Trends in Analytical Chemistry, 118, 722-730 (2019). http://dx.doi.org/10.1016/j.trac.2019.06.028.

Zhang C*, Bills BJ, Manicke NE. Rapid prototyping using 3D printing in bioanalytical research. Bioanalysis, 9 (4), 329-331 (2017). Invited editorial. http://dx.doi.org/10.4155/bio-2016-0293.

Manicke NE*, Bills BJ, Zhang C. “Analysis of Biofluids by Paper spray MS: Advances and Challenges.” Bioanalysis, 8 (6), 589-606 (2016). Review article. http://dx.doi.org/10.4155/bio-2015-0018

Liu J, Manicke NE, Zhou X, Cooks RG, Ouyang Z: Paper Spray. In: New Developments in Mass Spectrometry, Domin M and Cody R (Eds). Royal Society of Chemistry, 389-422 (2015). Book Chapter

Liu J, Manicke NE, Cooks RG, Ouyang Z: Paper Spray Ionization for Direct Analysis of Dried Blood Spots. In: Dried Blood Spots: Applications and Techniques. Li W and Lee MS (Eds). John Wiley & Sons, Inc., 298-313 (2014). Book Chapter